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Representative images from immunofluorescence staining of co-localization between phospho-histone H2A.X and anti-53BP1 antibodies in HeLa, MCF7, CNE-2 and HCT116 cells treated with fresh medium (FM) or harvested conditioned medium (CM) having previously conditioned the bystander cells (partnered with IRCs) that were incubated until 12 h post-irradiation and immunofluorescent staining was performed. Scale bar = 50 μm.
