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. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: Virology. 2018 Mar 22;518:301–312. doi: 10.1016/j.virol.2018.03.009

Fig. 5. Lysosomotropic agents block ZIKV infection.

Fig. 5.

Ammonium Chloride, Chloroquine and Bafilomycin A1, inhibit ZIKV infection. VERO and Cf2Th cells were challenged with ZIKV-RVPs or ZIKV-MR766 at the indicated MOI in the presence of increasing concentrations of ammonium Chloride (A), Chloroquine (B) or Bafilomycin A1(C). All drugs were used at concentrations that were not toxic to cells. Forty-eight hours post-challenge infection was determined by measuring the percentage of GFP-positive or 4G2-positive cells for ZIKV-RVPs or ZIKV-MR766 infections, respectively. As a control, similar experiments were performed using the pH-dependent pseudo-typed virus HIV-1-VSV-G. Experiments were repeated at least three times and a representative experiment is shown. P < 0.05 (*), P < 0.01 (**), P < 0.001 (***) or not significant (ns), using two-tailed Student’s t-test are shown. U: not infected and not treated.