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. 2020 Jun 17;9:e56910. doi: 10.7554/eLife.56910

Figure 3. CypA is required for MxB restriction.

Figure 3.

(a) HIV-1 GFP WT or P90A vector titre in HEK293 untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)). (b) HIV-1 GFP P90A RT products, 2LTR circles and proviral DNA in HEK293 untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)). (c) HIV-1 GFP vector titre, RT products, 2LTR circles in HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) and depleted of CypA by shRNA transduction. (d) Matched representative immunoblot analysis from (c) detecting MxB or CypA. Tubulin detected as loading control. (e) HIV-1 GFP vector titre on HEK293 untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) after treatment with CsA at the point of infection. (f) HIV-1 GFP WT (left) or P90A (right) vector titre on HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) after CsA was added at the stated times after infection. Titre of HIV-1 GFP vector containing the stated CA mutants in. (g) HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)),. (h) HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) and depleted of CypA by shRNA transduction or. (i, j) HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) after treatment with CsA at the point of infection. + / - SEM n = 3. (***) p<0.01 (**) p<0.05 (*) p<0.1 (ns) not significant p>0.1.