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. 2020 Jun 17;9:e56910. doi: 10.7554/eLife.56910

Figure 4. CypA induced HIV-1 capsid conformational change is required for MxB restriction.

Figure 4.

(a) Titre and (b) fold MxB restriction of HIV-1 GFP vector, containing gag-pro sequences from the stated isolates, in HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) after CsA was added at the point of infection. (c) Summary of virus phenotypes, amino acid sequence, and conservation around the CypA binding loop of the stated isolates. (d) Titre of HIV-1-GFP wild type or indicated mutant in HEK293 cells untreated (UT) or treated with Dox to induce MxB expression (+Dox (MxB)) after CsA was added at the point of infection. (e) Structure of HIV-1 CA protein (PDB: 6ES8) indicating interaction of residues M96, G116 and H120. + / - SEM n = 3. (***) p<0.01 (**) p<0.05 (*) p<0.1 (ns) not significant p>0.1.