Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Staphylococus lugdunensis) | N940135 | National Reference Center for Staphylococci, Lyon, France (Heilbronner et al., 2011) | ||
| Strain, strain background (S. lugdunensis) | N920143 | National Reference Center for Staphylococci, Lyon, France (Heilbronner et al., 2011) |
||
| Strain, strain background (S. lugdunensis) | N920143 ΔisdEFL | This paper | Markerless deletion mutant of isdEFL | |
| Strain, strain background (S. lugdunensis) | N920143 ΔlhaSTA | This paper | Markerless deletion mutant of lhaSTA | |
| Strain, strain background (S. lugdunensis) | N920143 ΔisdEFLΔlhaSTA | This paper | Markerless double deletion mutant of isdEFL and lhaSTA | |
| Cell line (Human) | Human cardiac myocytes (HCM) | PromoCell | C-12810 | |
| Recombinant DNA reagent | pQE-30 | Qiagen | IPTG inducible expression plasmid | |
| Recombinant DNA reagent | pQE30:lhaS | This paper | LhaS expressing plasmid for protein purification | |
| Recombinant DNA reagent | pRB473: lhaSTA | This paper | LhaSTA expressing plasmid for complementation | |
| Recombinant DNA reagent | pIMAY (plasmid) | Monk et al., 2012 | See Material and methods | Thermosensitive vector for allelic exchange |
| Recombinant DNA reagent | pIMAY:∆isd | Zapotoczna et al., 2012 | Plasmid for the deletion of the entire isd locus | |
| Recombinant DNA reagent | pIMAY:∆isdEFL | This study | Plasmid for the deletion of conventional heme transporter isdEFL | |
| Recombinant DNA reagent | pIMAY:∆lhaSTA | This study | Plasmid for the deletion of heme specific ECF-transporter | |
| Recombinant DNA reagent | pRB473 | Brückner, 1992 | Expression plasmid without promotor region. | |
| Biological sample (Human) | Human hemoglobin | Own preparation | See Material and methods | Sex male |
| Biological sample (Pork) | Porcine hemin | Sigma | 51280 | |
| Biological sample (Human) | Human Myoglobin | Sigma Aldrich | M6036 | |
| Biological sample (Horse) | Equine Myoglobin | Sigma Alrich | M1882 | |
| Biological sample (Human) | Human Haptoglobin (Phenotype 1–1) | Sigma Aldrich | SRP6507 | |
| Biological sample (Human) | Human Hemopexin | Sigma Aldrich | H9291 | |
| Chemical compound, drug | RPMI 1640 Medium | Sigma Aldrich | R6504-10L | |
| Chemical compound, drug | Casamino acids | BACTO | 223050 | |
| Chemical compound, drug | EDDHA | LGC Standarts | TRC-E335100-10MG | |
| Chemical compound, drug | Dodecyl-β-D-maltosid (DDM) | Carl Roth | CN26.1 | |
| Chemical compound, drug | 3,3',5,5'-tetramethylbenzidine (TMBZ) | Sigma Aldrich | 860336 | |
| Chemical compound, drug | Profinity IMAC resin nickel chrged | BIO RAD | 1560135 |