Skip to main content
. 2020 Jan 10;9:e50973. doi: 10.7554/eLife.50973

Figure 1. An in vitro assay for mitochondrial inner-membrane fusion.

Figure 1.

(A) Mitochondrial membrane fusion involves sequential outer and inner membrane fusion. The mitofusins (Mfn1/2) catalyze outer membrane fusion. In metazoans, mitochondrial inner-membrane fusion is mediated by Opa1. (B) Linear domain arrangement of l-Opa1. (C) Schema of the experimental setup. (D) Fusion assay. Membrane tethering, docking, lipid mixing, and content release can be distinguished using fluorescent reporters that specifically reflect each transition of the reaction.