Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | 18:1 (Δ9-Cis) PC (DOPC) | Avanti Polar lipids | Cat #: 850375P | |
| Chemical compound, drug | 1',3'-bis[1,2-dioleoyl-sn-glycero-3-phospho]-glycerol (sodium salt) | Avanti Polar lipids | Cat #: 710335P | |
| Chemical compound, drug | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)−2000] (ammonium salt) | Avanti Polar lipids | Cat #: 880130P | |
| Chemical compound, drug | L-α-lysophosphatidylinositol (Liver, Bovine) (sodium salt) | Avanti Polar lipids | Cat #: 850091P | |
| Chemical compound, drug | 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine | Avanti Polar lipids | Cat #: 850757P | |
| Chemical compound, drug | Texas Red 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine, Triethylammonium Salt (Texas Red DHPE) | ThermoFisher Scientific | Cat #: T1395MP | |
| Chemical compound, drug | 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(Cyanine 5) | Avanti polar lipid | Cat #: 810335C1mg | |
| Chemical compound, drug | Calcein | Sigma-Aldrich | Cat #: C0875; PubChem Substance ID: 24892279 | |
| Strain | Pichia pastoris SMD1163 (his4,pep, prb1) | Rapoport lab; Harvard Medical School. | ||
| Recombinant DNA reagent | pPICZ A-TwinStrep-lOPA1-H10 | GenScript | plasmid to transform and express human WT l-Opa1 (isoform1). | |
| Recombinant DNA reagent | pPICZ A-TwinStrep-sOPA1-H10 | GenScript | plasmid to transform and express human WT s-Opa1 (s1). | |
| Recombinant DNA reagent | pPICZ A-TwinStrep-lOPA1 (G300E)-H10 | GenScript | plasmid to transform and express G300E mutant of l-Opa1 (isoform 1). | |
| Recombinant DNA reagent | pPICZ A-TwinStrep-sOPA1 (G300E)-H10 | GenScript | plasmid to transform and express G300E mutant of s-Opa1 (s1). | |
| Antibody | Rabbit Anti-Opa1 antibody | NOVUS BIOLOGICALS | Cat #: NBP2-59770 | Western Blot 2 ug/ml |
| Antibody | Mouse 6x-His Tag Monoclonal Antibody (HIS.H8) | ThermoFisher Scientific | Cat #: MA1-21315 | Western Blot 1:2000 |
| Antibody | Mouse StrepMAB-Classic, HRP conjugate (2-1509-001) | IBA Lifesciences | Cat #: 2-1509-001 | Western Blot 1:2500/1:32000 |
| Antibody | Rabbit IgG HRP Linked Whole Ab | SIGMA-ALDRICH INC | Cat #: GENA934-1ML | |
| Antibody | Mouse IgG HRP Linked Whole Ab | SIGMA-ALDRICH INC | Cat #: GENA931-1ML | |
| Chemical compound, drug | GTP Disodium salt | SIGMA-ALDRICH INC | Cat #: 10106399001 | |
| Commercial assay, kit | EnzChek Phosphate Assay Kit | ThermoFisher Scientific | Cat #: E6646 | |
| Chemical compound, drug | GppCp (Gmppcp), Guanosine-5'-[(β,γ)-methyleno]triphosphate, Sodium salt | Jena Bioscience | Cat #: NU-402–5 | |
| Chemical compound, drug | n-Dodecyl-β-D-Maltopyranoside | Anatrace | Cat #: D310 25 GM | |
| Chemical compound, drug | n-Octyl-α-D-Glucopyranoside | Anatrace | Cat #: O311HA 25 GM | |
| Chemical compound, drug | Lauryl Maltose Neopentyl Glycol (LMNG) | Anatrace | Cat #: NG310 | |
| Chemical compound, drug | LMNG-CHS Pre-made solution | Anatrace | Cat #: NG310-CH210 | |
| Chemical compound, drug | Zeocin | Invivogen | Cat #: ant-zn-1p | |
| Chemical compound, drug | Ni-NTA | Biorad | Cat #: 7800812 | |
| Chemical compound, drug | StrepTactin XT | IBA Lifesciences | Cat #: 2-4026-001 | |
| Chemical compound, drug | Biotin | IBA Lifesciences | Cat #: 2-1016-005 | |
| Chemical compound, drug | Superose 6 Increase 10/300 GL | GE | Cat #: 29091596 | |
| Chemical compound, drug | TEV protease | Prepared in lab, purchased from GenScript | Cat #: Z03030 | |
| Chemical compound, drug | Benzonase Nuclease | Sigma-Aldrich | Cat #: E1014 | |
| Chemical compound, drug | Protease inhibitor cocktail | Roche | Cat #: 05056489001 | |
| Chemical compound, drug | Leupeptin | Sigma-Aldrich | Cat #: L2884 | |
| Chemical compound, drug | Pepstatin A | Sigma-Aldrich | Cat #: P5318 | |
| Chemical compound, drug | Benzamidine hydrochloride hydrate | Sigma-Aldrich | Cat #: B6506 | |
| Chemical compound, drug | Phenylmethylsulfonyl fluoride (PMSF) | Sigma-Aldrich | Cat #: 10837091001 | |
| Chemical compound, drug | Aprotinin | Sigma-Aldrich | Cat #: A1153 | |
| Chemical compound, drug | Trypsin inhibitor | Sigma-Aldrich | Cat #: T9128 | |
| Chemical compound, drug | Bestatin | GoldBio | Cat #: B-915–100 | |
| Chemical compound, drug | E-64 | GoldBio | Cat #: E-064–25 | |
| Chemical compound, drug | Phosphoramidon disodium salt | Sigma-Aldrich | Cat #: R7385 | |
| Commercial assay, kit | 3–12% Bis-Tris Protein Gels | ThermoFisher Scientific | BN1003BOX | |
| Commercial assay, kit | NativePAGE Running Buffer Kit | ThermoFisher Scientific | BN2007 | |
| Commercial assay, kit | NativePAGE 5% G-250 Sample Additive | ThermoFisher Scientific | BN2004 | |
| Commercial assay, kit | NativePAGE Sample Buffer (4X) | ThermoFisher Scientific | BN2003 | |
| Software, algorithm | Slidebook | Intelligent imaging | RRID: SCR_014300 | |
| Software, algorithm | Fiji/ImageJ | Fiji | SCR_002285 | |
| Software, algorithm | FCS analysis tool | Smith Lab, University of Akron |