Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Caenorhabditis elegans, hermaphrodite) | N2 wild type | Wormbase | WB Cat# N2_(ancestral), RRID:WB-STRAIN:N2_(ancestral) | |
| Genetic reagent (C. elegans, hermaphrodite) | wdr-5 (ok1417) III | Wormbase | WB Cat# RB1304; RRID:WB-STRAIN:RB1304 | |
| Genetic reagent (C. elegans, hermaphrodite) | met-2 (n4256) III | Wormbase | WB Cat# MT13293; RRID:WB-STRAIN:MT13293 | |
| Genetic reagent (C. elegans, hermaphrodite) | jhdm-1 (ok2364) III | Wormbase | WB Cat# RB1826, RRID:WB-STRAIN:RB1826 | |
| Genetic reagent (C. elegans, hermaphrodite) | wdr-5 (ok1417) met-2 (n4256)/qC1 qIs26 [lag-2::GFP + pRF4 rol-6(su1006)] III | this paper | recombinant chromosome III isolated by crossing MT13293 and RB1304, then crossed to KW2203 to maintain over qC1 balancer | |
| Antibody | Mouse monoclonal anti-H3K9me2 | Abcam | Abcam:ab1220; RRID:AB_449854 | WB: 1:500 |
| Antibody | Mouse monoclonal anti-actin | Millipore/Upstate | Millipore:MAB1501; RRID:AB_2223041 | WB: 1:5000 |
| Antibody | rabbit polyclonal secondary anti-mouse IgG (HRP) | Abcam | Abcam:ab6728; RRID:AB_955440 | WB: 1:3000 |
| Commercial assay or kit |
ECL Plus | Amersham Biosciences | Amersham Biosciences:RPN2106 | |
| Commercial assay or kit | Chromatin Immunoprecipitation Assay Kit | EMD Millipore | EMD Millipore:17–295 | |
| Commercial assay or kit | iO SYBR Green Supermix | Bio-Rad | Bio-Rad:1708882 | |
| Software | Bowtie2 | PMID: 22388286 | RRID:SCR_005476 | |
| Software | MACS v2.1.1 | PMID: 22936215 | RRID:SCR_013291 | |
| Software | deepTools2 | PMID: 27079975 | RRID:SCR_016366 | |
| Software | Integrated Genome Viewer (IGV) | PMID: 22517427 | ||
| Software | SeqPlots | PMID: 27918597 | ||
| Software | BEDtools v2.27.1 | PMID: 20110278 | RRID:SCR_006646 |