Figure 8. Growth parameters of the 12 DM25-evolved clones and their 3 Cit⁺ ancestors.

(A) Estimates of growth parameters for each ancestral and DM25-evolved clone, using the log-slope method (Figure 2). Estimates for ancestral strain CZB151 and its descendants are shown in black, estimates for CZB152 and its descendants are in orange, and estimates for CZB154 and its descendants are in blue. Units for growth rates r are h⁻¹, and units for lag times are h. Bias-corrected and accelerated (BC_a) bootstrap 95% confidence intervals around parameter estimates were calculated using 10,000 bootstraps; no confidence interval is shown if a parameter could not be estimated accurately from the available data. Aberrant estimates that fall outside of these ranges are not shown. (B) Estimates of log₂-transformed ratios of growth parameters for the evolved clones and their ancestors. The growth curves used to estimate these parameters are shown in Figure 8—figure supplements 1 and 2.

Figure 8—figure supplement 1. Growth curves of the 12 DM25-evolved clones, measured in DM25 only.

(Many DM25-evolved clones grew inconsistently in DM0.) For comparison, growth curves of the evolved clones are paired with those of their founders: CZB151 (top row), CZB152 (middle row), and CZB154 (bottom row). The evolved and ancestral curves are shown in purple and gray, respectively.

Figure 8—figure supplement 2. Log_e-transformed growth curves of the 12 DM25-evolved clones, measured in DM25.

Dashed black and red lines indicate the intervals we used to calculate growth rates on glucose and citrate, respectively (Figure 2). See Figure 8—figure supplement 1 for additional details.