Table 2. Copy number of amplified maeA and dctA genes in sequenced clones from populations that evolved for 2500 generations in either DM0 or DM25 environments.

Genome	Medium	Gene	Mean copy number	Minimum number*	Maximum number*	Adjusted p-value†
ZDBp880	DM0	dctA	2.33	1.67	3.26	<0.0001
ZDBp886	DM0	dctA	3.20	1.88	4.25	<0.0001
ZDBp898	DM0	dctA‡	2.09	1.71	2.76	0.0023
ZDBp913	DM25	dctA	3.17	1.91	4.68	<0.0001
ZDBp918	DM25	dctA	3.41	1.80	5.19	<0.0001
ZDBp919	DM25	dctA	2.60	2.06	3.29	<0.0001
ZDBp883	DM0	maeA	58.47	22.61	95.46	<0.0001
ZDBp889	DM0	maeA	34.71	2.35	55.39	<0.0001
ZDBp904	DM0	maeA	28.08	15.30	44.72	<0.0001
ZDBp911	DM25	maeA	2.22	1.54	3.39	<0.0001
ZDBp917	DM25	maeA	4.72	3.56	6.18	<0.0001
ZDBp919	DM25	maeA	12.81	2.16	18.00	<0.0001

^*These bounds indicate the ratio of the minimum and maximum sequencing coverage measured at the indicated locus to the mean sequencing coverage over the genome.

^†Significance levels are shown after Bonferroni corrections for multiple tests of the same hypothesis.

^‡There may be two discontinuous amplifications of dctA in this genome, or there may be a single continuous amplification with a short region of low coverage within the gene. The second region of amplification has a similar copy number. We present data for only one region, which provides a conservative estimate of the overall statistical significance in this case.