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. 2020 Jun 5;16(6):e1008799. doi: 10.1371/journal.pgen.1008799

Fig 6. Exogenous expression of TRF2 or TRF1 rescues BRM deficiency-induced telomere uncapping and replication defect.

Fig 6

(A) Western blot analysis of exogenous and endogenous TRF2 in BRM depleted cells. (B) Western blot analysis of exogenous and endogenous TRF1 in BRM depleted cells. (C) IF-FISH detection of γH2AX foci in control and BRM-depleted VA13 cells with EV or exogenous TRF2 expression. (D) Quantification of (C). Data represent the mean ± SEM of three independent experiments (n ≥ 100), **P < 0.01, ***P < 0.001. (E) IF-FISH detection of PCNA foci in control and BRM-depleted VA13 cells with EV or exogenous TRF1 expression. (F) Quantification of (E). Data represent the mean ± SEM of three independent experiments (n ≥ 100), **P < 0.01. (G) IF-FISH detection of RPA1 foci in control and BRM-depleted VA13 cells with EV or exogenous TRF1 expression. (H) Quantification of (G). Data represent the mean ± SEM of three independent experiments (n ≥ 100), ***P < 0.001, ****P < 0.0001.