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. 2020 Jun 5;16(6):e1008799. doi: 10.1371/journal.pgen.1008799

Fig 7. Exogenous expression of TRF2 and TRF1 rescues BRM deficiency-induced genome instability and cell apoptosis.

Fig 7

(A) Western blot analysis of exogenous TRF2 and TRF1 expression and BRM knockdown efficiency. (B) Detection of micronuclei in control and BRM-depleted VA13 cells with EV or exogenous TRF2 or/and TRF1 expression. (C) Quantification of the micronuclei in (B), the fraction of cells with micronuclei were calculated. Data represent the mean ± SEM of three independent experiments (n ≥ 200 cells), ****P < 0.0001. (D) FACS analysis of apoptotic cells in control and BRM-depleted VA13 cells with EV or exogenous TRF2 or/and TRF1 expression. (E) Quantification of the apoptotic ratio in (D). Data represent the mean ± SEM of three independent experiments, ***P < 0.001, ****P < 0.0001.