FIGURE 3.

(A) Hypoxyprobe staining of white matter. (B) Overlay of Visiopharm labeling. Green represents hypoxyprobe staining; blue represents normoxic white matter in the region of interest. Scale bar, 100 µm. (C) Interrater reliability. Visomorph quantification of pimondazole staining of ipsilateral corpus callosum and external capsule by 2 blinded operators. R² =  0.9953. (D) Test–retest reliability. Visiomorph quantification of pimonidazole staining of ipsilateral corpus callosum and external capsule. Two sets of 12 slices spaced 300 μm apart were stained for pimonidazole binding in the ipsilateral corpus callosum and external capsule and quantified by blinded operators. R² =  0.9597. Each data point in (C) and (D) represents quantification of 12 slices spaced 300 μm apart from a single brain.