Table 1:
Global fitted parameters. The global fit incorporates all fluorescence-detected thermal melts and kinetics of all oligomers simultaneously. One standard deviation errors are shown in parentheses. Supplementary Information section 2 discusses each parameter in detail: One effective melting temperature, five thermal and denaturant linear free energy parameters, four fluorescence baseline parameters, and two activation free energy parameters. The parameters for state M are missing in the fit with only two states per domain, which has a much worse error (χ2).
| Parameters | 3 states per domain model | 2 states per domain model |
|---|---|---|
| Tm (°C) | 82.4 (0.5) | 82.0 (0.6) |
| g(0)UM (J/mol−1) | 213 (100) | - |
| gUN (J mol−1 K−1) | 377 (15) | 290 (12) |
| gUM (J mol−1 K−1) | 163 (20) | - |
| mUN (J/mol−1 molar−1) | 3145(200) | 2060 (138) |
| mUM (J/mol−1 molar−1) | 1140 (300) | - |
| bU | 358.7 (0.2) | 358.1 (0.3) |
| aU | −0.03 (0.02) | −0.10 (0.02) |
| bF | 347.1 (0.2) | 346.4 (0.3) |
| aF | 0.011 (0.001) | 0.008 (0.005) |
| GϯNU (J/mol−1)* | 18500 (8000) | 19900 (1300) |
| GϯMU (J/mol−1)* | 2600 (fixed) | - |
| Avg. χ2 (thermo)** | 1.7 | 23 |
| Avg. χ2 (kinetics)** | 1.5 | 3.1 |
*
GϯNU and GϯMU are barrier height going from folded to unfolded or intermediate to unfolded form
**
16 thermal melts with 288 data points total and 30 kinetic decays each with 674 data points