Figure 7.

The expression of sirtuins in the carotid arteries and VSMCs. A‐C, The expression levels of SIRT1, SIRT3, SIRT5 and SIRT6 in carotid arteries were determined by Western blotting and real‐time PCR, respectively. D, E, The VSMCs were treated with TGF‐β1 (1.25, 2.5, 5, 10 and 20 ng/mL) for 24 h, and the expression levels of SIRT1, SIRT3, SIRT5 and SIRT6 in VSMCs were determined by Western blotting. Statistical significance was determined using ANOVA by Student's t test. *P < 0.05 and **P < 0.01 vs. the control group. (F and G) The effect of miR‐145 on the expression of SIRT1, SIRT3, SIRT5 and SIRT6 in VSMCs was determined by Western blotting. Statistical significance was determined using ANOVA by Tukey's post hoc test. **P < 0.01 vs. the LV‐NC group. ^# P < 0.05 and ^## P < 0.01 vs. the LV‐NC + TGF‐β1 group. The results are expressed as the mean ± SEM, n = 3