FIGURE 5.

Melatonin regulates organelle stress responses induced by acidosis. A, The differentially expressed proteins related to organelle stress are listed with a ratio (pH6.2/Con), including ATP synthase F (0) complex subunit C2, mitochondrial (ATP5G2), carbamoyl phosphate synthetase 1 (CPS1), phosphatidylserine synthase 1 (PSS1) and midkine (MDK). Red colour indicates the increased proteins (P < 0.05), and green indicates the decreased ones (P < 0.05). Levels of Beclin1, microtubule‐associated protein light chain 3 (LC3), ras‐related protein rab‐7a (Rab7), lysosomal‐associated membrane protein 1 (LAMP1), LAMP2, glucose‐regulated protein 78 (GRP78/Bip) and activating transcription factor 6 (ATF6) were measured by Western blotting (B) and quantitatively analysed (C). Levels of Golgi matrix proteins including trans‐Golgi network protein 46 (TGN46), Golgi reassembly stacking protein 55 (GRASP55), GRASP65 and Golgi matrix proteins golgin‐84 (Gol‐84) were measured by Western blotting (D) and quantitatively analysed (E). Data were presented as means ± SEM (n = 4/group). *P < 0.05, **P < 0.01, *** P < 0.001 vs Con, ^# P < 0.05, ^## P < 0.01 vs Veh