TABLE 1.
Antibodies employed in this study and their properties
| Antibody | Epitopes | mAb/pAb | Dilution | Source |
|---|---|---|---|---|
| MAP2 | Total MAP2 | pAb | 1:1000 (IF) | Abcam (Cambridge, UK) |
| NF‐κB p65 | p‐NF‐κB p65 at Ser536 | pAb | 1:1000 (WB) | Cell Signaling Technology (Danvers, MA, USA) |
| p‐Nrf2 | p‐Nrf2 at Ser40 | pAb | 1:1000 (WB) | Abcam |
| DM1A | α‐tubulin | mAb | 1:2000 (WB) | Abcam |
| NR2A | Total NR2A | pAb | 1:1000 (WB) | Cell Signaling Technology |
| NR2B | Total NR2B | pAb | 1:1000 (WB) | Cell Signaling Technology |
| NR1 | Total NR1 | pAb | 1:1000 (WB) | Millipore (Billerica, MA, USA) |
| GluR1 | Total GluR1 | pAb | 1:1000 (WB) | Millipore |
| GluR2 | Total GluR2 | mAb | 1:1000 (WB) | Millipore |
| Synapsin 1 | Total Total synapsin 1 | pAb | 1:1000 (WB) | Millipore |
| Syntaxin1 | Total syntaxin 1a | pAb | 1:1000 (WB) | Abcam |
| Synaptophysin | Total synaptophysin | pAb | 1:1000 (WB) | Abcam |
| SNAP25 | Total SNAP25 | mAb | 1:1000 (WB) | Cell Signaling Technology |
| VAMP3 | Total VAMP3 | pAb | 1:1000 (WB) | Thermo Fisher Scientific |
| PSD95 | Total PSD95 | pAb | 1:500 (WB) | Cell Signaling Technology |
| Tau‐5 | Total tau | mAb | 1:1000 (WB) | Abcam |
| pT231 | p‐tau at Thr231 | pAb | 1:1000 (WB) | Signalway Antibody |
| pS396 | p‐tau at Ser396 | mAb | 1:1000 (WB) | Cell Signaling Technology |
| ps214 | p‐tau at Ser214 | pAb | 1:1000 (WB) | Signalway Antibody |
| pS404 | p‐tau at Ser404 | pAb | 1:1000 (WB) | Signalway Antibody |
| pT205 | p‐tau at Thr205 | pAb | 1:1000 (WB) | Cell Signaling Technology |
| Tau‐1 | dep‐tau at Ser198/199/202 | mAb | 1:1000 (WB) | Millipore |
| t‐GSK3β | Total GSK‐3β | pAb | 1:1000 (WB) | Cell Signaling Technology |
| pGSK3β | p‐GSK‐3β at Ser9 | pAb | 1:1000 (WB) | Cell Signaling Technology |
| t‐AKT | Total AKT | pAb | 1:1000 (WB) | Cell Signaling Technology |
| p‐AKT | p‐AKT at Ser473 | pAb | 1:1000 (WB) | Cell Signaling Technology |
| ERK | Total MAPK ERK1/2 | pAb | 1:1000 (WB) | Cell Signaling Technology |
| p‐ERK | p‐ERK at Thr202/Tyr204 | pAb | 1:1000 (WB) | Cell Signaling Technology |
| PP2Ac | PP2A catalytic subunit | pAb | 1:1000 (WB) | Biosource (Camarillo, CA, USA) |
| p‐PP2Ac | p‐PP2A at Tyr307 | pAb | 1:1000 (WB) | Santa Cruz (Dallas, TX, USA) |
| Beclin 1 | Total Beclin 1 | pAb | 1:1000 (WB) | Abcam |
| LC3 | Total LC3 | mAb | 1:1000 (WB) | Cell Signaling Technology |
| Rab7 | Total Rab7 | mAb | 1:1000 (WB) | Cell Signaling Technology |
| Lamp1 | Total Total Lamp1 | mAb | 1:1000 (WB) | Abcam |
| Lamp2 | Total Lamp2 | mAb | 1:1000 (WB) | Abcam |
| GRP78 | Total GRP78 | pAb | 1:1000 (WB) | Abcam |
| ATF6 | Total ATF6 | pAb | 1:1000 (WB) | Abcam |
| TGN46 | Total TGN46 | mAb | 1:1000 (WB) | Abcam |
| GRASP55 | Total GRASP55 | mAb | 1:1000 (WB) | Santa Cruz |
| GRASP65 | Total GRASP65 | mAb | 1:1000 (WB) | Abcam |
| Golgin 84 | Total golgin 84 | mAb | 1:1000 (WB) | Santa Cruz |
| Cas3 | Total caspase‐3 | mAb | 1:1000 (WB) | Cell Signaling Technology |
| C‐cas3 | Total cleaved caspase‐3 | mAb | 1:1000 (WB) | Cell Signaling Technology |
Abbreviations: IF, immunofluorescence; mAb‐, monoclonal antibody; p‐, phosphorylated; pAb‐, polyclonal antibody; WB, Western blotting.