FIGURE 2.

AdHIP/PAP alleviates BLM‐induced lung injury and inflammation in mice. Mice in the AdHIP/PAP + BLM group lost less body weight than those in the other two BLM groups. ^* P < 0.05 vs the NS + BLM or AdGFP + BLM groups and P < 0.01, vs the NS group (A). Similarly, AdHIP/PAP significantly alleviated the increase in the mouse lung coefficient on both day 3 and day 28 after BLM installation (B). Adenovirus‐mediated HIP/PAP expression obviously mitigated lung injury and inflammation, as indicated by H&E staining of mouse lungs (C), BALF protein concentration determination (D), and cell counting (E) on day 3 after BLM instillation consistently showed that the anti‐inflammatory action of HIP/PAP is substantiated by the decreased expression of CD45 in mouse lung, as indicated by qRT‐PCR and immunohistochemistry results (F, G). Error bars indicate SD. Scale bars = 100 μm