Figure 1.

Sal B protected viability of HEI‐OC1 cells upon neomycin and cisplatin toxicity. (A) Effect of neomycin (Neo) on HEI‐OC1 cells. HEI‐OC1 cells were treated with or without various concentrations of Neo (1‐30 mM) for 24 hours. (B) Effect of Sal B on neomycin‐induced viability in HEI‐OC1 cells. Cells were pre‐treated with or without various concentrations of Sal B (10‐50 μM) for 2 hours followed by 10 mM neomycin co‐treatment for 24 hours except those in the non‐treated control (Control) group. (C) Effect of cisplatin (Cis) on HEI‐OC1 cells. (D) Effect of Sal B on cisplatin‐induced ototoxicity in HEI‐OC1 cells. Cell viability was measured by CCK‐8 kit. Values were represented as the mean ± SEM from three independent experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001, and ns. not significant vs the non‐treated control group; ^## P < 0.01, ^### P < 0.001 and ^#### P < 0.0001 vs the group treated with 10 mM neomycin or 30 μM cisplatin only