Figure 7.

Sal B activated the PI3K/AKT pathway in HEI‐OC1 cells undergoing neomycin‐ and cisplatin‐induced apoptosis. (A) Effect of Sal B on the expression of phospho‐PI3K (P‐PI3K) and phospho‐AKT (P‐AKT) in neomycin‐ and cisplatin‐damaged HEI‐OC1 cells. (B‐E) HEI‐OC1 cells were pre‐treated with Sal B (40 μM) and LY294002 (10 or 20 μM) or MK‐2206 (1 or 10 μM) for 2 hours and then exposed to 10 mM neomycin or 30 μM cisplatin for 24 hours. Cell viability was quantified by CCK‐8 assay. Data were presented as the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001 and ns. not significant vs the group treated with 10 mM neomycin or 30 μM cisplatin only; ^# P < 0.05, ^### P < 0.001 and ^#### P < 0.0001 vs the group treated with Sal B and neomycin or cisplatin. Scale bars = 20 μm