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. 2020 May 5;24(12):6690–6703. doi: 10.1111/jcmm.15321

FIGURE 5.

FIGURE 5

Effect of APS‐IgG and inhibitors on Akt, ERK1/2 and p38 MAPK phosphorylation in neutrophils. Neutrophils isolated from healthy pregnant women were treated with APS‐IgG (15 µug/mL), HC‐IgG (15 µg/mL) and PMA (50 nmol/L) or left untreated (NT) for 3 h. A‐C, Western blotting of p‐Akt (ser473), p‐ERK1/2 (Thr202/Tyr204) and p‐p38 MAPK (Thr180/Tyr182) expression. Neutrophils were separately pre‐treated with the inhibitors MK2206 (10 μmol/L), SCH772984 (10 μmol/L) or SB203580 (20 μmol/L) for 0.5 h, followed by 3 h of incubation with APS‐IgG (15 µg/mL) or complete medium (NT). D‐F, Western blotting of p‐Akt, p‐ERK1/2 and p‐p38 MAPK expression. Data are presented as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01; ns, not significant. APS, antiphospholipid syndrome; HC, healthy control