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. 2020 May 5;24(12):6690–6703. doi: 10.1111/jcmm.15321

FIGURE 7.

FIGURE 7

NETs induced by APS‐IgG have detrimental effects on the invasion and migration of trophoblasts as well as the migration and tube formation ability of HUVECs. HTR‐8/SVneo cells and HUVECs were treated with cell‐free NETs for 12 h. A, Representative images of Transwell invasion and migration assays. Scale bars = 50 microns. B, Values represent mean ± SEM (n = 3 independent experiments) of the number of cells per field. C, Representative images of wound‐healing assays at 0 h and 24 h. Scale bars = 200 microns. D, Values represent mean ± SEM (n = 3 independent experiments) of cell migrated area. For the tube formation assay, an additional group consisted of HUVECs incubated with DNase I (10 U/mL, 37℃ and 1 h). E, Representative images of Transwell migration assay. Scale bars = 50 microns. F, Values represent mean ± SEM (n = 3 independent experiments) of the number of cells per field. G, Representative images of tube formation assay at 8 h. Scale bars = 200 microns. H, Values represent mean ± SEM (n = 3 independent experiments) of the number of tube branches, junctions, meshes and mesh area. **P < 0.01; ns, not significant. APS, antiphospholipid syndrome; HC, healthy control; NET, Neutrophil extracellular trap