Figure 2.

The changes of pulmonary surfactant protein expression, glycogen content and the interaction between C/EBPα and TGFβ2 following SUMO1 knockdown in BPD rats. Three neonatal rats per group were selected were killed from newborn rats at P14 and one sample repeated three times in our experiment. Comparison of the expression levels of pulmonary surfactant proteins in the rat lungs among control, si‐NC and si‐SUMO1 groups (A). Periodic acid and Schiff (PAS) staining assay on rat lung tissues from the different groups (B). Immunofluorescence imaging; C/EBPα was partially co‐localized with TGFβ2 in the rat lung tissues (C). Co‐immunoprecipitation assay (Co‐IP); the levels of co‐immunoprecipitated C/EBPα and TGFβ2 expression in different groups (D). Scale bar = 20 µm; original magnifications: ×200; square frame magnification: ×400. Values are presented as mean ± SD; ** P < .05 vs control