FIGURE 5.

SOX3 promotes gastric cancer cell invasion and migration through MMP9. A, SOX3 silence exerted little effects on cell proliferation of MKN45 (P = .867) and AGS cells (P = .653). B and C, 3D invasion assays showed that SOX3 silence repressed MKN45 invasion significantly (P = .028). D and E, zPDX studies indicated that SOX3 silence repressed MKN45 migration in vivo on day 3 post‐injection (dpi) (P = .015) (MKN45 and MKN45‐SOX^low cells were shown with red fluoresce using the DiL staining). F and G, MMP9 expression was inhibited with SOX3 silence (P = .024 for MKN45 and P = .018 for AGS). H, Chromatin immunoprecipitation‐PCR assay showed SOX3 was the transcription factor responsible for MMP9 expression. zPDx, patient‐derived xenograft model in zebrafish