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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Cancer Res. 2020 Mar 10;80(12):2484–2497. doi: 10.1158/0008-5472.CAN-19-1892

Figure 3.

Figure 3.

TAp63-regulated miR-30c-2* and miR-497–5p suppresses cuSCC through induction of cell death and cell cycle arrest. A-B, SYBR green qRTPCR of TAp63 (A) and Dicer (B) in NHEKs following transfection with the indicated siRNAs. C-D, Taqman qRTPCR of miR-30c-2* C, and miR-497–5p D in NHEKs following transfection with the indicated siRNAs. E, Representative growth curve of nucRed-mCherry-labeled COLO16 cells transfected with the indicated microRNA mimics. F-G, Immunofluorescence (F) and quantification (G) for Annexin V-488 (green)-positive nucRed-mCherry-labeled COLO16 cells transfected with the indicated microRNA mimics. H-I, Immunofluorescence (H) images and quantification (I) for Edu (green) incorporation in COLO16 cells transfected with the indicated microRNA mimics following a 3 hour Edu pulse. NucRed® dead 647 (red) was used as a counterstain. J-K, Cell cycle profiles (J) and quantification (K) of COLO16 cells 48 hours after transfection with the indicated microRNA mimic as measured by FACS analysis. M phase was measured as the percentage of cells staining positive for Histone H3-pS28-AF647. Data shown are mean ± SD, n=3, unless noted otherwise. * p<0.05, ** p<0.01, ***p<0.001, Student’s t test (two-tailed).