Figure 7:

miR-30c-2* and miR-497 suppress cuSCC growth in vivo. A, COLO16 cells stably expressing RFP and luciferase were transfected with the indicated microRNA mimics and subcutaneously injected into both flanks of athymic nu/nu mice. B, Representative images of mice injected with COLO16 cells transfected with scrambled, miR-30c-2*, and miR-497–5p microRNA mimics on Day 19 prior to tumor harvest. C, Tumor volume assessed using caliper s. D, Time course of in vivo bioluminescence imaging of tumor xenografts. E, Quantification of extracted tumor volumes. Data shown are mean ± SEM, n=10. * p<0.05, Student’s t test (two-tailed).