Figure 3.
LAC*0914::tn and LAC*glnQ::tn strains do not show a defect in glutamine or glutamate uptake. Amino acid uptake assays. (a, b) S. aureus strains LAC* (WT), LAC*0914::tn and LAC*alsT::tn were grown to mid‐log phase in GDM + Glu+NH3. Subsequently, radiolabelled (a) glutamine or (b) glutamate was added to culture aliquots, samples removed and filtered at the indicated time points and the radioactivity accumulated in the cells measured. The average values and SDs from three experiments were plotted. (c, d) Amino acid uptake assays were performed and the data plotted as described in panels (a) and (b), but using S. aureus strains LAC* (WT) and LAC*glnQ::tn (glnQ::tn)