Figure 6.

Addition of glutamine and ammonium but not glutamate stimulates the growth of S. aureus in glucose‐containing defined medium. (a) Bacterial growth curves. The WT S. aureus strain LAC* was grown in 96‐well plates in glucose‐containing defined medium (GDM) or in GDM supplemented with glutamine (Gln), glutamate (Glu), ammonium (NH₃) or combinations thereof as specified in the legend. OD₆₀₀ readings were determined every 30 min and the average and SDs of three biological replicates plotted. (b) Bacterial growth curves. S. aureus strains LAC* piTET (WT piTET), the alsT mutant strain LAC*alsT::tn piTET and the complementation strain LAC*alsT::tn piTET‐alsT were grown in GDM + Gln medium supplemented with 200 ng/µl Atet. OD₆₀₀ readings were determined every 30 min and the average and SDs of three biological replicates plotted