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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: J Pediatr Surg. 2020 Feb 27;55(6):1107–1112. doi: 10.1016/j.jpedsurg.2020.02.038

Figure 1.

Figure 1.

Structural adaptation and ECM enrichment of ileal tissue from sham surgery vs 50% proximal SBR mice. A. We controlled for metabolic factors such as age, gender, caging, and weight of mice. Graph depicts weights of sham vs SBR animals on post-operative day (POD) 0 (day of surgery) and 7 (time of tissue harvest). B. At POD 7 after either sham surgery or 50% proximal SBR, ileal tissue 2cm distal to the anastomosis was harvested and submitted for histological analysis to confirm structural adaptation (ie villus lengthening) in SBR mice (P< 0.05). Representative images of ileum from sham and SBR mice at post-operative day 7 are shown (40x, scale bar: 100 μm). B. Enrichment for ECM (which was extracted from flash-frozen ileal tissue using the CNMCS kit) was verified using Western Blotting for proteins expected within each fractionated component. C= cytoplasmic, N= nuclear, M= membrane, CS= cytoskeletal.