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. Author manuscript; available in PMC: 2020 Nov 25.
Published in final edited form as: Nat Plants. 2020 May 25;6(6):686–698. doi: 10.1038/s41477-020-0666-7

Extended Data Fig. 8. BAP treatment functions through cytokinin signaling.

Extended Data Fig. 8

a, 5 μM BAP treatment on the TCS cytokinin signaling reporter (gray) for 24 hours. Control showing that cytokinin treatment enhances TCS reporter expression. Chlorophyll autofluorescence: red. Scale bars: 50 μm. n = 3 inflorescences for each treatment.

b, 5 μM BAP treatment on the cytokinin receptor mutant wol-1 for 4 days. Control showing that mutation of the cytokinin receptor (wol-1) abrogates delayed sepal initiation in response to cytokinin. Lower left flower removed during imaging. Cell walls stained with PI: gray. Scale bar: 50 μm. n = 3 inflorescences.

c, NAA (auxin) treatment in a gradient of concentration on the TCS cytokinin signaling reporter (gray) for 24 hours. Auxin treatment did not enhance TCS reporter expression. Control showing that the induction of TCS reporter expression is specific to cytokinin treatment. Chlorophyll autofluorescence: red. Scale bars: 20 μm. n = 3 inflorescences for each treatment.