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. Author manuscript; available in PMC: 2020 Nov 25.
Published in final edited form as: Nat Plants. 2020 May 25;6(6):686–698. doi: 10.1038/s41477-020-0666-7

Extended Data Fig. 1. drmy1–2 floral organs have enhanced sepal size and shape variability.

Extended Data Fig. 1

a, DRMY1 mutations have little effect on the floral organ number. Numbers of sepals, petals, stamens and carpels were quantified for both WT and the drmy1–2 mutant. Two-tailed Student’s t test * p-value < 0.05 (p-value for the mean of organ number, WT versus drmy1–2 petal: 1.14E-03; WT versus drmy1–2 stamen number: 2.14E-08). Measure of centre: mean. Error bars: standard error of the mean. n = 30 flowers.

b, Quantification of the mean sepal area of the four sepals from an individual flower. Sequential flowers along the main branch of the stem (flower number on the x-axis) were measured at stage 14. Three replicates were included for both WT (blue) and drmy1–2 (red). Original individual sample curves can be found in Source Data. The mean of the 3 replicates are presented as thick blue and red lines with the SD as partially translucent background.

c, Quantification of the sepal shape variability for outer, inner and lateral sepals. Two-tailed Student’s t test * p-value < 0.05 (p-value for the mean of shape variability, WT versus drmy1–2 inner sepal: 2.70E-02; WT versus drmy1–2 lateral sepal: 1.00E-07) Measure of centre: mean. Error bars: standard error of the mean. n = 60 for both WT and drmy1–2 10th to 25th flowers along the main branch.

d, What at first appeared to be two sepals initiated at the inner sepal position of the drmy1–2 flower (left panel) fused to form a single sepal with a split tip at later time points of the live imaging (right panel). Red arrowheads: initiating sepals. Red arrow: the fused sepal. Scale bar: 20 μm.

e, Coefficient of variation (CV) calculated for the areas of the outer, inner and lateral sepals. Sepals from different flowers were pooled together. n = 48 flowers.

f, Average CV calculated for the areas of all petals in each single flower for WT and drmy1–2. Two-tailed Student’s t test * p-value < 0.05 (p-value for the mean of CV for petal area within individual flowers, WT versus drmy1–2: 1.27E-03). Measure of centre: mean. Error bars: standard error of the mean. n = 20 flowers for both WT and drmy1–2.