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Chromatographic conditions for studying chemical stability of insulin using RP-HPLC.
| Column | Thermo Scientific™ Hypersil GOLD™ C18 column (250 × 4.6 mm, 5 μm) |
| Mobile phase A | 0.1% v/v TFA in deionized water |
| Mobile phase B | 0.1% v/v TFA in acetonitrile |
| Elution | Gradient |
| Mobile phase composition | 75:25 (A:B) to 50:50 (A:B) |
| Flow rate | 0.8 ml/min |
| Injection volume | 25 μl |
| Run time | 25 min |
| Detector, Detection wavelength | UV, 280 nm |
