. Author manuscript; available in PMC: 2021 Jul 10.

Published in final edited form as: J Control Release. 2020 Apr 10;323:161–178. doi: 10.1016/j.jconrel.2020.04.012

Table 4.

Midpoint transition temperature (Tm) and transition enthalpy (ΔH) of insulin, zinc-insulin hexamers and chitosan-zinc-insulin complexes prepared using oleic acid-grafted chitosan polymers, in phosphate buffered saline at pH 7.4. Data are expressed as mean ± S.D, n=3.

Sample	Tm₁ (°C)	Tm₂ (°C)	AH (kJ/mol)
Insulin	51.87 ± 0.23	62.81 ± 0.29	97.27 ± 0.44
Zinc-Insulin hexamers	72.57 ± 0.19	-	344.15 ± 0.62
CSO (5 kDa) –Zinc-Insulin complex	86.73 ± 0.07	-	572.88 ± 4.33
Oleic acid (25%)-g-CSO-Zinc-Insulin complexes	86.53± 0.17	-	459.53 ± 5.29
Oleic acid (45%)-g-CSO-Zinc-Insulin complexes	86.30 ± 0.10	-	449.51 ± 3.21