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. 2020 Jun 17;7:182. doi: 10.1038/s41597-020-0509-4

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© The Author(s) 2020

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Fig. 2 — Stereocilia and gold bead segmentation workflow using Amira software package. (a–d), Stereocilia segmentation. The membrane of each stereocilium was outlined with a brush tool as shown in a every few sections depending on the orientation of the section through the cilium (b), then selections were ‘filled’ within respective section frames using the ‘fill holes’ function (c), and interpolated through the sections in between to segment the entire stereocilium (d). (e) Amira screen view with all voxels comprising a single stereocilium selected. (f) Amira screen view illustrating all stereocilia segmented within the bundle. Note only three rows of stereocilia were segmented, leaving the remaining shorter stereocilia out. (g) Amira screen view illustrating all gold beads segmented on the surface of the bundle.