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. 2020 Jun 17;7:182. doi: 10.1038/s41597-020-0509-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Screen capture of Amira project at the step of surface rendering. Following segmentation, each stereocilia bundle used in this study was subsequently rendered using the ‘Generate surface’ function of Amira for visualization purposes. The results are also provided in a movie illustrating 3D localization of PKHD1L1 in immunogold labeled outer hair cell stereocilia bundles using FIB‐ SEM, available at figshare²⁷.