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. 2020 Jun 17;10:9800. doi: 10.1038/s41598-020-66704-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Formation of in vitro oral biofilms obtained from saliva samples from a healthy (a) and a periodontal (b) donor with and without the AHL-lactonase Aii20J (20 µg/mL) using the Active Attachment model and measured with the crystal violet staining assay (O.D. 590 nm). The cultures were done in aerobic conditions using BHI and BHI supplemented with 0.1% sucrose (BHIs) for 24 h (n = 3) and in anaerobic conditions for 24 and 48 h using BHI (n = 1). Significantly different values are indicated with an asterisk (Student’s t-test, p < 0,05).