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. 2020 Jun 17;10:9800. doi: 10.1038/s41598-020-66704-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal microscopy visualization (63×) of the effect of the QQ enzyme Aii20J (20 µg/mL) on multi-species biofilms formed by the oral pathogens A. naeslundii, A. actinomycetemcomitans, F. nucleatum, P. gingivalis S. oralis and V. parvula using fluorescence dyes Syto9 and propidium iodide. The biofilms were cultured in BHI-2 during 3 and 4 days in anaerobic conditions at 37 °C.