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. 2020 Jun 17;10:9832. doi: 10.1038/s41598-020-66785-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunophenotyping of tumor cells within fabricated constructs and clinical sample. Samples from constructs cultured in vitro, constructs implanted in vivo, and clinical CRC biopsies of varying grade were immune-stained for markers related to EMT and oncogenesis, as indicated. Staining results were analyzed using Visiopharm and graphed as the proportion of cancer cells with: (a) nuclear localization of Ki67; (b) with fully intact, membrane localized E-Cadherin expression; (c) with N-Cadherin expression; (d) with nuclear localization of β-Catenin. (e) Ratio of total area corresponding to positive FAK expression in tumor spheroid and clinical CRC biopsies of varying grade. Graphs represent mean + s.e.m. of three regions of interest from each sample of experiments performed in triplicate.