Fig. 5.

Increased PTEN activation in rTg4510 mice, preceding caspase-3 cleavage. a Schematic depicting negative regulation of PTEN activity by phosphorylation. b Representative western blots and quantification of pPTEN (inactive) and total PTEN using extracts from the hippocampus of 2, 4, and 6 month-old WT and rTg4510 mice reveal an increase in total PTEN levels relative to the inactive form at 2 and 4 months. c Representative western blots and quantification of pPTEN (inactive) and total PTEN of synaptosomal lysates from the hippocampus of 2, 4, and 6 month-old WT and rTg4510 mice, revealing an increase in total PTEN relative to the inactive form at 4 and 6 months. Data presented as mean ± SEM, *p < 0.05, **p < 0.01, b, c unpaired t test performed between WT and rTg4510 at each age group independently