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. 2020 Jun 1;23(6):101217. doi: 10.1016/j.isci.2020.101217

Figure 3.

Figure 3

CCL8 Is Tumor Promoting during Involution

(A) Growth of MCF10.DCIS.com (2x105 cells) in SCID (wt) and Ccl8KOSCID (Ccl8KO) mice implanted in about 3-month nulliparous mice (n = 6). Results are shown as average +SEM.

(B) Growth of MCF10.DCIS.com (2x105 cells) in SCID (wt) and Ccl8KOSCID (Ccl8KO) mice implanted in day 2 of forced involution that followed 10 days of lactation (n = 6). The inset shows tumor onset in different groups. Results are shown as average + SEM (∗, p < 0.05). See also Figure S2.

(C) Same as (B) with macrophage depletion (n = 5) starting from lactation day 7, using five daily intraperitoneal injections of 1 mg clodronate liposomes. See also Figures S3 and S4.

(D) Hematoxylin and eosin (H&E) in tumors from wt or Ccl8KO nulliparous mice or mice implanted in involution (n = 6). Scale bar, 20 μm in 20x. N, nulliparous; PR, pregnancy; LAC, lactation; INV, involution.