Fig. 1.

The base editing effect using pXMJ19-dCas9 1 plasmid in C. glutamicum. (A) Structure and schematic diagram of plasmid pXMJ19-dCas9 1 used for single base editing. (B) Single base editing efficiency of different 20 PAMS. (C) Structure and sequence of the Ptrc-sgRNA expression cassette. (D) Secondary structure of the gRNA formed by the excess sequence downstream of the Ptrc promoter with 20N and the sgRNA scaffold. The base of the 20 ​nt target sequence is highlighted by a blue sphere. (E) 20 ​nt target sequence and the secondary structure of the gRNA formed by the downstream sgRNA scaffold. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)