Table 1.
Primers used in this study.
| Primer | Sequence (5’→3’) |
|---|---|
| DAF | ACAATTTCACACAGGAAACAGAATTAATTAAAAGGAGGACAACTAATGGATAAAAAGTATTCCA |
| DAR | CCGCCAAAACAGCCAAGCTGTCACAGGCCCAGGGTGCG |
| D10AF | GGCCTGGCTATCGGCACCAATTC |
| D10AR | GCCGATAGCCAGGCCAATGGAATAC |
| H840AF | GTGGACGCCATCGTCCCTCAGTC |
| H840AR | GACGATGGCGTCCACATCGTAATCAGAG |
| VectorsgRNA.F | CATTTCGAAGTGAGTTAGCGCGAATTGATCTGGTTTG |
| VectorsgRNA.R | TTATTGGTGCCCTTCGAAGAAGCCGCACGT |
| FragmentsgRNA.F | CGGTCATTTCGAAGTGAGTTAGCGCG |
| FragmentsgRNA.R | GGCAGTTATTGGTGCCCTTCGAAGAAGCC |
| sg1F | CCGGAATTCGGATTCTCGTTGGTAGGTTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
| sg2F | CCGGAATTCGGAATTCCGTGAAAATGTTTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
| sg3F | CCGGAATTCGTTCCGCATCGACCACTATTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
| sg4F | CCGGAATTCGCTGCAGGCAGAAAAGATCAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC |
| sgR | TGCTCTAGAAAAAAAGCACCGACTCGGTGCCACTTTTTCAAG |
| zwfseq F | CGGCGACATGAAATCGAATTAGTTC |
| zwfseq R | GGGTGGTGGTATCCGGAAG |
| cas9F | TCCAGACAATTCCGACGTGGACAAG |
| cas9R | GGTTGGTTTTAAACAGGAGGTCGACG |
| J23100F | TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23102F | TTGACAGCTAGCTCAGTCCTAGGTACTGTGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23104F | TTGACAGCTAGCTCAGTCCTAGGTATTGTGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23105F | TTTACGGCTAGCTCAGTCCTAGGTACTATGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23108F | CTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23111F | TTGACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23113F | CTGATGGCTAGCTCAGTCCTAGGGATTATGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23118F | TTGACGGCTAGCTCAGTCCTAGGTATTGTGCTAGCAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| J23119F | TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTAGAAGAGCGCTCTTCAGTTTTAGAGCTAGAAATAGCAAG |
| JR | ATAAAACGAAAGGCCCAGTCT |
| F23100F | TGACAGCTTATCATTTGACGGCTAGCTCAGTCC |
| F23102F | TTTGACAGCTTATCATTTGACAGCTAGCTCAGT |
| F23104F | ACAGCTTATCATTTGACAGCTAGCTCAGTC |
| F23105F | GACAGCTTATCATTTTACGGCTAGCTCAGTC |
| F23108F | ACAGCTTATCATCTGACAGCTAGCTCAGTCC |
| F23111F | TTGACAGCTTATCATTTGACGGCTAGCTCAGTC |
| F23113F | ACAGCTTATCATCTGATGGCTAGCTCAGTCC |
| F23118F | ACAGCTTATCATTTGACGGCTAGCTCAGTCC |
| F23119F | AGCTTATCATTTGACAGCTAGCTCAGTC |
| F231R | GAGCGTTCACCGACAAACAACAGATAAAACGAAAGGC |
| V231F | CCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTC |
| V23100R | GCTAGCCGTCAAATGATAAGCTGTCAAACCAGAT |
| V23102R | AGCTAGCTGTCAAATGATAAGCTGTCAAACCAGAT |
| V23104R | CTAGCTGTCAAATGATAAGCTGTCAAACCA |
| V23105R | GCTAGCCGTAAAATGATAAGCTGTCAAACCA |
| V23108R | AGCTAGCTGTCAGATGATAAGCTGTCAAACCAG |
| V23111R | CTAGCCGTCAAATGATAAGCTGTCAAACC |
| V23113R | GCTAGCCATCAGATGATAAGCTGTCAAACCA |
| V23118R | GAGCTAGCCGTCAAATGATAAGCTGTCAA |
| V23119R | AGCTAGCTGTCAAATGATAAGCTGTCAAACCA |
| zwfsg4F | AGTGCTGCAGGCAGAAAAGATCA |
| zwfsg4R | AACTGATCTTTTCTGCCTGCAGC |
| FtadaF | TCCGGATTATGCAATGAAACGGACAGCCGACGGAAG |
| FtadaR | ATACTTTTTATCCATTGACCCCCCGCTG |
| VtadaF | GGGCAGCAGCGGGGGGTCAATGGATAAAAAGT |
| VtadaR | GCTGTCCGTTTCATTGCATAATCCGGAAC |