Fig. 3.

The bz1 mutant displays impaired AGPs biosynthesis. a Comparison of monosaccharides composition of mature stem between wild-type (WT) and bz1 plants. * and ** indicates significant differences between WT and bz1 by t-test at P < 0.05 and 0.01, respectively. b Immunohistochemical analysis of AGPs in culm sections using JIM13 and LM2 monoclonal antibodies. The bz1 shows substantially weaker signals than WT in all cells including the vascular bundle, sclerenchyma and parenchyma cells. c Staining of hand-cut stem sections with βGlcY and βManY (control). The bz1 shows weaker signal than the WT. d Immunogold labeling of sclerenchyma and parenchyma cell walls of WT and bz1 plants with JIM13 and LM2 monoclonal antibodies. e The bz1 shows fewer particles in both sclerenchyma and parenchyma cell walls. f Comparison of the abundance of AGPs and its monosaccharides composition in WT and bz1. The AGPs content was measured and calculated as μg g^− 1 fresh weight of tissue. The results are means ± SE of three independent assays