FIGURE 4.

Quantification of fission and fusion proteins. (A) Representative images of ATP synthase β (mitochondrial area) and DRP1 (quantification) signals from one control subject (C4) and one PARK2-mutated patient (P2). The merge panel shows the juxtaposition of the two signals. Insets show the magnification (165%) of details in squares. (B) Quantification of the DRP1 signal per mitochondrial surface unit. Data were expressed as mean ± SEM. ^∗p = 0.026. (C) Representative western blot image of MFN1 protein in primary skin fibroblasts of PARK2-mutated patients (P1, P2, P3, P4, and P5) and control subjects (C1, C2, C3, C4, and C5). (D) Relative quantification expressed as mean ± SEM. Data were analyzed by two-tailed, unpaired Student’s t-test by comparison of PD patients and control subjects. (E) Representative western blot image of OPA1 protein in primary skin fibroblasts of PARK2-mutated patients (P1, P2, P3, P4, and P5) and control subjects (C1, C2, C3, C4, and C5). (F) Relative quantification expressed as mean ± SEM. Data were analyzed by two-tailed, unpaired Student’s t-test by comparison of PD patients and control subjects.