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. 2020 Jun 11;8:423. doi: 10.3389/fcell.2020.00423

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Copyright © 2020 Zilocchi, Colugnat, Lualdi, Meduri, Marini, Corasolla Carregari, Moutaoufik, Phanse, Pieroni, Babu, Garavaglia, Fasano and Alberio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein-protein interaction alterations. (A) PPI-network of altered proteins in the mitochondrial fraction. Purple nodes, strictly mitochondrial proteins; light green nodes, mitochondria-associated proteins. (B–D) Co-IP experiments of indicated prey proteins (HSPA8, HSPD1, and ALDH2, red line in panel (A) in mitochondrial-enriched fractions obtained from fibroblasts of C4 control subject and P3 PARK2-mutated subject using specific antibodies. Protein G beads served as a negative control. Blots were probed using HSPA8 (B), HSPD1 (C), and ALDH2 (D) antibodies.