FIGURE 3.

Thermodynamic and colloidal stability assessment of Pre-F, Post-F, Hex G, and Pre-F/G constructs. (A) Differential scanning calorimetry; based on the T_m (transition midpoint), the thermodynamic stability of the individual domains can be ranked Post-F>> Hex G > Pre-F, and Pre-F/G. (B) Dynamic light scattering indicates a similar degree of colloidal stability for Hex G, Pre-F and Pre-F/G, while Post-F is extremely colloidally stable, as indicated by lack of change in size within the temperature range of the experiment. (C) Table summarizing the conformational transitions (A) and colloidal stability (B) of Pre-F, Post-F, Hex G, and Pre-F/G.