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. 2020 Jun 11;11:842. doi: 10.3389/fimmu.2020.00842

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Copyright © 2020 Loomis, Stewart-Jones, Tsybovsky, Caringal, Morabito, McLellan, Chamberlain, Nugent, Hutchinson, Kueltzo, Mascola and Graham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunogenicity of NiV Pre-F stabilized immunogens, multimeric forms of G and Pre-F/G chimeric immunogens. (A,B) Recognition of pre-F (A) or post-F (B) NiV F proteins by sera from mice immunized twice with NiV F designs or unimmunized. (C,D) Recognition of pre-F NiV F (C) or mono G (D) protein by sera from mice immunized twice with NiV F, NiV G multimers or NiV F/G chimeric designs or unimmunized. (E) Recognition of pre-F NiV F or mono G proteins by sera from mice immunized twice with NiV Hex G or NiV Stalk G. Binding kinetics were measured using a fortéBio Octet Red384 instrument. Line represents mean of all animals in each group ± standard deviation (using GraphPad Prism).