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. 2020 Jun 11;11:842. doi: 10.3389/fimmu.2020.00842

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Copyright © 2020 Loomis, Stewart-Jones, Tsybovsky, Caringal, Morabito, McLellan, Chamberlain, Nugent, Hutchinson, Kueltzo, Mascola and Graham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neutralization of NiVF/G VSVΔG-luciferase pseudovirus by sera from mice immunized with NiV stabilized pre-F, multimeric forms of G and F/G chimeric immunogens. VSVΔG-luciferase pseudovirus (expresses both NiV F_WT and NiV G on surface) neutralization assays were performed on individual mouse sera collected at week 5. The log₁₀ reciprocal IC₈₀ titer for each sample was calculated by curve fitting and non-linear regression using GraphPad Prism. P-values were calculated using one-way ANOVA with Tukey’s multiple comparisons test (***p < 0.001, ****p < 0.0001). Line represents mean of log₁₀ reciprocal IC₈₀ titer ± standard deviation (using GraphPad Prism).