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. 2020 Jun 11;14:644. doi: 10.3389/fnins.2020.00644

FIGURE 2.

FIGURE 2

Histopathological studies of WWOX-deficient human brain. (A) H&E staining shows an incorrect migration of the external granular layer within the molecular layer in patient II.3 (right panel) compared to a normal fetus (left panel) at the same gestational age. Images at 10X and 20X magnification (molecular layer: ML, external granular layer: EGL, cortical gray: CG). Scale bar: 50 μm. (B) Histological staining with GFAP 10× (upper panel) and 20× (lower panel) in which a disorganization of the irregularly distributed trajectories is observed (black arrows). Scale bars: 50 μm. (C) Histological staining with CD-31 at 10× (upper panel) and 20× (lower panel), in which thinning of the vessels is observed in the context of the external granular layer (black arrows). (D) Histological staining with Shift Reactive Periodic Acid (PAS) at 10× (upper panel) and 20× (lower panel) show that the vascular structures of the cortex are irregularly distributed and irregularly branched (black arrows). Scale bars 50 μm.