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. 2020 Jun 11;10:865. doi: 10.3389/fonc.2020.00865

Figure 3.

Figure 3

UCHL5 knockdown decreased cell viability, increased apoptosis, and arrested cell cycle in HCE-1-A endometrial cancer cells. (A) The levels of UCHL5 were detected by WB in HCE-1-A cells at 48 h after shRNA lentiviral infection (N = 3 times). *P < 0.05 vs. control. (B) Relative mRNA levels of UCHL5 in shRNA lentiviral infected HCE-1-A cells (N = 3). *P < 0.05 vs. control. (C) Relative cell viability was detected by CCK-8 at different time points (N = 3). *P < 0.05 vs. sh-UCHL5-1 and sh-UCHL5-2. (D) Apoptosis cells were marked by Annexin V-FITC/PI, and the percentage was analyzed by flow cytometry in HCE-1-A cells at 48 h after shRNA lentiviral infection (N ≥ 3). *P < 0.05 vs. sh-NC (negative control). (E) Cell cycle was measured by Cell Cycle Analysis Kit (N ≥ 3). *P < 0.05 vs. sh-NC.